Title
ENZYMATIC ABILITIES AND COMPARISON OF LIPOLYTIC ACTIVITY OF TWO YARROWIA LIPOLYTICA STRAINS
Autor
Anna Gdula, Józefa Chrzanowska, Marek Szołtysik, Maria Wojtatowicz, Maria Elisabetta
Keywords
Yarrowia lipolytica, enzyme profile, lipolytic activity, butter fat
Abstract
Enzymatic activities (extra- and intracellular) of two strains of Yarrowia lipolytica yeast: A-101 and W29, originating from soil of different locations, were analysed by API-ZYM test. Additionally the lipolytic activity of these strains was compared. This activity was investigated by diffusion test on Spirit Blue Agar at different temperature (25 and 4ºC) and water activity (aw: 0.99; 0.97; 0.93) and by measuring the free fatty acids liberation during growth of both strains on butter fat. Both strains appeared to be similar in their enzyme profiles and had a capability to grow on butter and to release preferencially of oleic acid from milk fat.
Pages
83-89
Cite
Gdula, A., Chrzanowska, J., Szołtysik, M., Wojtatowicz, M., Elisabetta, M. (2003). ENZYMATIC ABILITIES AND COMPARISON OF LIPOLYTIC ACTIVITY OF TWO YARROWIA LIPOLYTICA STRAINS. Acta Sci. Pol. Biotechnol., 2(1-2), 83-89.
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