Title
In vitro regeneration induced in leaf explants of Citrus limon L. Burm cv. ‘Primofiore’
Autor
Ewa Szczuka, Jacek Pietrusiewicz, Anna Kasprzyk-Pawelec
Keywords
Citrus limon, phytohormones, explants, callus induction, auxin, cytokinin, 6-benzyloaminopurine
Abstract
In vitro organogenesis was studied using Citrus limon L. Burm cv. ‘Primofiore’ leaf explants. The purpose of the present study was to optimize conditions for callogenesis and organogenesis of C. limon. Explants of C. limon were cultured on 16 different media supplemented with various combinations of plant growth regulators, both auxins and cytokinins, such as 6-benzylaminopurine (BAP), naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin. The best shoot induction was obtained when the leaf explants were cultured on Murashige & Tucker media supplemented with 3.5 mg∙L-1 BAP. Histological investigation revealed most likely the initial phase of development of leaf explants during in vitro regeneration of C. limon.
Pages
143-153
Cite
Szczuka, E., Pietrusiewicz, J., Kasprzyk-Pawelec, A. (2015). In vitro regeneration induced in leaf explants of Citrus limon L. Burm cv. ‘Primofiore’. Acta Sci. Pol. Hortorum Cultus, 14(4), 143-153.
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