Title
YEAST SYNTHETASES OF ACETYL-COA: COMPARISON BY BIOINFORMATIC TOOLS
Autor
Mateusz Kropiwnicki, Joanna Koniuszewska, Małgorzata Robak
Keywords
Key words. Acetyl-CoA synthetase, ACS, bioinformatics, aminoacid sequences, nucleotide sequences, motif III
Abstract
The purpose of study was to compare acetyl-CoA synthetases (ASC1, ACS2) in yeast: Ashbya gossipii, Candida albicans, Candida glabrata, Debaromyces hansenii, Kluyveromyces lactis, Saccharomyces cerevisiae, Zygosaccharomyces bailii and ACSA in Schizosaccharomyces pombe. The first step was to compare nucleotide sequence of genes and aminoacid sequence of enzymes. For nucleotide sequences two different clusters of genes were obtained (ACS1 and ACS2) located between them ACSA gene from S. pombe. For aminoacid sequences obtained results were similar to nucleotide ones. In the next step, comparison of amino acid sequences to the sequence of ACS2 from Z. bailii, three conservative fragments were detected. Moreover the presence of motif III (consider as catalytically important) was confirmed. Five sequences were found (FTAGD, YFTGD, YFAGD, LRTGD, YFSGD) and their exact positions were determined.
Pages
5-16
Cite
Kropiwnicki, M., Koniuszewska, J., Robak, M. (2015). YEAST SYNTHETASES OF ACETYL-COA: COMPARISON BY BIOINFORMATIC TOOLS. Acta Sci. Pol. Biotechnol., 14(1), 5-16.
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