The high potential of Clostridium strains to synthetize industrially useful metabolites is known for a long time. However, their metabolic abilities are poorly understood. Clostridium bifermentans is a good example of such a Clostridium species. In the literature there is a lot of incoherent information about the ability of these bacteria to saccharide utilization. Simultaneously, there is no data about the metabolites which they product. In this work we demonstrated the metabolic pathways of selected saccharides in 11 strains of C. bifermentans isolated from samples collected from the natural environment, as well as in the reference strain, C. bifermentans ATCC 638. As a result, it was find out that bacteria are able to synthetize organic acids (succinic, lactic, formic, and acetic) as well as ethanol from selected saccharides. However, diols (1,3-propanediol and 2,3-butanediol) are not synthetized from saccharides by C. bifermentans. As earlier studies have shown diols are synthetized only from glycerol in the investigated strains.

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