Lignocellulosic biomass is widely available renewable raw material, which is an alternative for fossil fuels. The main direction of the development of the lignocellulosic materials is feeding and subsequently the production of biofuels, of which main step is degradation to free sugars. Six strains of Trichoderma fungi were assessed in terms of capability for the use of biodegradable atypical lignocellulosic waste, which is brewer’s spent grain (BSG). It was determined by the activity evaluation of five hydrolytic enzymes, and particular attention was paid to the concentration of reducing sugars released during and after propagation. High activity of fungal hydrolases was obtained in brewer’s spent grain medium. Free sugars (as equivalent of reducing sugars) reached much higher level, as compared to the pure BSG extract. The obtained results suggest a high potential of BSG as by-product to produce intermediate raw material with a high concentration of sugars by biological methods.

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