The production of selected extracellular hydrolytic enzymes by Trichoderma citrinoviride C-1 strain under various submerged culture conditions was examined. Production of cellulases (CMCases, FPases) and xylanases was observed in medium supplemented with lignocellulosic waste (2%). In turn, cell wall biopolymers of inactivated Fusarium biomass (3%) and inactivated fodder yeast biomass (3%) were used as carbon sources and inducers of chitinases and β-1,3-glucanases. The biosynthesis efficiency of both enzyme groups was dependent mostly on the main carbon source used in culture medium. The highest production of CMCases (1.17 U×ml^-1) and xylanases (7.29 U×ml^-1) was recorded on the 12th day of cultivation in mineral medium with lignocellulosic waste in the absence of glucose. In the present study medium supplementation with glucose was also investigated. The addition of glucose had a significant influence on cellulases and xylanases production which decreased with the increasing sugar concentration. Furthermore, T. citrinoviride C-1 strain’s capability to secretion of β-1,3-glucanases and chitinases was established and maximum biosynthesis was observed after 5 days (1.77 U×ml^-1 and 1.17 nKat×ml^-1 respectively) and decreased after this time. Application of waste materials, connected with high productivity of selected hydrolytic enzymes by T. citrinoviride, could significantly reduce the cost of their production.

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