Vitex agnus-castus L. (chaste tree) is a native plant species carrying valuable ornamental, functional and medicinal properties. It was aimed to determine the most practical and optimum rooting conditions for successful vegetative production of V. agnus-castus. It was focused on definition of optimal cutting collection time, and specification of the effects of different rooting substrates and IBA concentrations on rooting abilities and growth of V. agnus-castus cuttings. Two rooting experiments (Exp.) were performed; at the beginning and end of its deciduous season. Sand and sand:sphagnum peat (1:1 v/v) mixture were tested as substrate in Exp. I, and perlite in addition to former substrates in Exp. II. The hardwood stem cuttings were treated with different concentrations of IBA in both experiments. The results of Exp. II indicated both substrate and concentration, and also their interaction had significant effects on adventitious rooting formation. In addition, it was revealed the maximum rooting ratio (100%) and the most vigorous growth generated in V. agnus-castus cuttings collected at the end of its deciduous season and treated IBA concentrations in perlit medium.

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