Protein engineering is now a very important sector in modern biotechnology. They are used random mutagenesis, which allows production enzymes and proteins with the desired characteristics or increased activity. These techniques and the appropriate method of bacterial selection permit to accelerate the evolution of genomes for industry. There are many techniques to create random changes in the amino acid sequence of the protein. This can be achieved by treating the whole bacteria or DNA chemicals or UV radiation, using modified techniques of PCR or RCA in error-prone mix, mutator strains and using one of the methods of DNA shuffling. The following article provides a description of these methods and examples of successful use of them.

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