Acta Scientiarum Polonorum

Scientific paper founded in 2001 year by Polish agricultural universities

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Biotechnologia
(Biotechnologia) 15 (2) 2016
Title
TRICHODERMA LYTIC ENZYMES INDUCED BY YARROWIA LYPOLYTICA CELL WALL BIOPOLIMERS
Autor
Michał Piegza, Danuta Witkowska, Wojciech Łaba, Ewelina Siepka
Keywords
Trichoderma sp., Yarrowia lipolytica, mycoparasitism, chitinases, laminarinases
Abstract
The presented research was to assess the level of lytic enzymes biosynthesis by Trichoderma strains in the presence of Yarrowia lipolytica yeast biomass. Two strains of filamentous fungi: Trichoderma harzianum T33 and T. citrinoviride C1, as well as two strains of yeast Yarrowia lipolytica: 8665 UV’s and Y. lipolytica A101 were used. Grown-up Yarrowia biomass were inoculated with filamentous fungi spores and cultivated in the same time (during 8-days). The assessment of the outcoming culture fluids included the activity of lytic enzymes: chitinases, laminarinases and lichenases, and the level of extracellular proteins. The concentration of released proteins in most variants was low, however, for thermally inactivated Yarrowia biomass it reached best results (170–210 mg×ml-1). In most cases the presence of yeasts biomass had a positive effect on the the efficiency of lytic enzymes biosynthesis.
Pages
31-42
Cite
Piegza, M., Witkowska, D., Łaba, W., Siepka, E. (2016). TRICHODERMA LYTIC ENZYMES INDUCED BY YARROWIA LYPOLYTICA CELL WALL BIOPOLIMERS. Acta Sci. Pol. Biotechnol., 15(2), 31-42.
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