Hyacinthus genus is an important group of ornamental plants that bear white, yellow, pink, red or purple coloured flowers. It has about 2000 species spread around the world that are grown commercially. Although, plant occurs naturally in Turkey yet efforts have not been made to adapt it for open field cultivation. There is need to transfer and establish these plants from wild to fields for commercial use through in vitro and ex vitro approaches, that will help local economy profitably. This study reports in vitro culture of Hyacinthus orientalis L. subsp. orientalis; using fruits containing immature zygotic embryos cultured on MS medium containing varying concentrations of Thidiazuron (TDZ) with and without 0.2 mg l^-1 naphthaleneacetic acid (NAA) supplemented with 20 or 40 g l^-1 sucrose. The study also reports induction of bulblets on tender leaf sheaths on MS medium containing different concentrations of benzylaminopurine (BAP) + 0.1 mg l^-1 NAA supplemented with 30 g l^-1 sucrose. The maximum bulblet regeneration (40%) with 31.33 bulblets/explant was noted on MS medium containing 0.15 mg l^-1 TDZ supplemented with 40 g l^-1 sucrose. Whereas, the best bulblet regeneration on tender leaf sheath explants was noted on 1.5 mg l^-1 BAP + 0.1 mg l^-1 NAA with 2.97 bulblets per explant of 0.55 cm bulb diameter and 1.20 leaves per bulblet. These bulblets were cultured singly on MS medium containing 20 mg l^-1 GA₃ (Gibberellic acid) + 50 g l^-1 sucrose and attained a diameter of 0.75–1.00 cm in 30 days time. The bulbs regenerated on both explants were successfully rooted and acclimatised in plant growth chamber using peat moss followed by their transfer to open field conditions.

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