Artichoke is valued as a vegetable and medicinal plant. The aim of the study was to determine the effect of irrigation and leaf harvest date of artichoke grown in south-eastern Poland on total yield and marketable fresh leaf yield, its structure and the content of biologically active substances: total phenolic acids, flavonoids and tannins. Irrigation contributed to an increase in fresh leaf yield, percentage of marketable yield in total yield, as well as the increase in tannin content, decrease in total phenolic acid content, and no effect on the change in total flavonoid content. The plant material harvested in September was characterized by a higher content of phenolic acids and a lower content of flavonoids compared to the raw material obtained in October.

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