One of the oldest medicinal plants in the world is thyme (Thymus vulgaris L.). On the biological value of this plant is affected by biotic and abiotic conditions, which include fertilizers and soil type. The aim of the present study was to determine the effect of organic fertilization and the type of substrate on the biological value of the thyme herb. Plants were grown in a greenhouse in a different type substrates: peat and peat + sand, using varying amounts of manure 10, 20, 30 g·dm^-3 of substrate. In the obtaining raw material was determined the content of essential oil, dry matter, L-ascorbic acid, total nitrogen and proteins. There was a significant increase in the N-total and protein contents in thyme herb after application of increasing doses of examined manure independently of the test substrate. Applied natural fertilization and type of substrate did not affect significantly the content of essential oil in Thymus vulgaris L. herb. The highest content of L-ascorbic acid and dry matter was obtained for thyme after application of the lowest dose of manure, regardless of the substrate used.

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