The numerous publications on the positive impact of sea buckthorn and its products on human health concern mainly the fruits and seeds. However, there is little data relating to the properties of sea buckthorn leaves. The main objective of this study was to evaluate the chemical composition and isotope content in the leaves of sea buckthorn Hippophae rhamnoides L. depending on where its existence. The habitat conditions significantly differentiated the studies traits. Mean crude protein content in the leaves reached 235.0 g∙kg^-1 d. m. The most proteins contain plants growing on native soil in the Dolna Odra Power Station. The ash content, fat, fiber and total carbohydrates was significantly higher in the leaves of sea buckthorn growing on sandy soil reclaimed from using ash from coal with NPK 60 – 70 – 70 + sludge. Their average concentration was: 49.2, 63.0, 128.1, 587.4 g∙kg^-1 d. m., respectively. The concentration of micronutrients was varied and depended significantly from the place of living plants. A higher level of δ¹⁵N found in sea buckthorn grown on land reclaimed and fertilized with NPK. The conditions living of sea buckthorn didn’t differentiate δ¹³C value. It cannot be unequivocally determined whether the results of the study on the influence of habitat conditions on chemical and isotope composition of sea buckthorn leaves are permanent. Therefore, it is necessary to continue the research.

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