Title
USE OF GLYCEROL AS A CARBON SOURCE FOR THE PRODUCTION OF YEAST BIOMASS OF CANDIDA UTILIS IN MONOCULTURE AND IN MIXED CULTURE WITH ACETIC BACTERIA GLUCONOBACTER OXYDANS
Autor
Stanisław Błażejak, Lidia Stasiak-Różańska, Agnieszka Kurcz, Anna Bzducha-Wróbel
Keywords
Candida utilis, Gluconobacter oxydans, glycerol, dihydroxyacetone
Abstract
In this study glycerol was used as a carbon source for the production of C. utilis biomass in monoculture and in mixed culture with G. oxydans. Yeast culture was performed in control media as well as growth media with glycerol concentration 30 gL-1. It also has been checked if dihydroxyacetone (DHA) can be used as a carbon source for C. utilis for biomass production. For this purpose yeast were cultivated with acetic bacetria (mixed culture) in growth media. Acetic bacteria are able to oxidate glycerol to DHA. During the cultivation optical density and biomass yield were measured. The highest increase of yeast biomass was observed in the control media after 24 hours and it was 15.8 g dry matter per L. After 48 hours of C. utilis culture in media contains glycerol (30 gL-1) the yield was 11,4 g d.m.L-1, while in mixed culture it was 12.5 g d.m.L-1. The highest yield of C. utilis biomass in media consist 2% of DHA was 2.7 g d.m.L-1 after 48 hours. The experiment has shown that DHA is not a easily assimilable carbon source for C. utilis.
Pages
5-14
Cite
Błażejak, S., Stasiak-Różańska, L., Kurcz, A., Bzducha-Wróbel, A. (2016). USE OF GLYCEROL AS A CARBON SOURCE FOR THE PRODUCTION OF YEAST BIOMASS OF CANDIDA UTILIS IN MONOCULTURE AND IN MIXED CULTURE WITH ACETIC BACTERIA GLUCONOBACTER OXYDANS. Acta Sci. Pol. Biotechnol., 15(3), 5-14.
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