Erythritol is a low calorie sucrose substitute, naturally occurring in fruit and honey. Erythritol is industrially produced by osmophilic yeast using fermentation process with glucose or sucrose and purification processes based on ion chromatography, decolorization and crystallization. High production costs from traditional carbon sources such as glucose and high costs of purification of erythritol cause to look for alternative low-cost technologies of sweetener production. The aim of this study was to develop the purification process of fermentation broth rich in erythritol, which was obtained from waste glycerol from biodiesel industry by Yarrowia lipolytica yeast. The parameters of purification process such as purity of crystals were investigated. As a result of two-stage crystallization process the crystals of erythritol with 99.5% of purity were obtained with the overall yield of the process of 91.5%. The costs of the production of erythritol might be decreased by using the fermentation process without salt addition and the purification without desalination.

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