The subject of the presented work were preliminary studies to obtain Trichoderma biopreparation, based on liquid culture medium. Cultures of filamentous fungi can be an alternative to solid state fermentation (SSF) in obtaining spore-based biopreparations, relevant in the protection of plants against phytopathogens. The study focused on the assessment of the biological value of 6 strains of Trichoderma cultured in liquid potato medium, complemented with lignocellulosic waste, such as beet, wheat bran and apple pomace. Out of the tested strains after the assessment of the hydrolytic activity level (cellulases, xylanases polygalacturonases, lichenases and laminarinases), 2 strains were selected for cultivation in bioreactors, intended for the obtaining of a biopreparation. After the cultures, biomass was centrifuged, along with the remains of the ligninocellulosic material, dried in a stream of hot air. Survival rate of spores was assessed, both, directly after drying and after 3 months of storage. The strain TRS40 was characterized by higher viability (72%) as compared with the TRS43 strain, which ensures activity of the biopreparation.

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