In the present day, the enzyme industry is looking for the highest activity. They can be obtained using methods of their mutagenesis. Site-directed mutagenesis techniques allow changes in a specific site in the amino acid sequence of proteins. These include the primer extension method, QuikChange PCR, MegaWhop PCR, GeneTailorT.Site-Directed Mutagenesis System, OmniChange PCR. These techniques can also be used to study the meaning of specific amino acids in the protein. In case we do not know which amino acids are best to replace, which would be best for the selected site, then we can use saturation mutagenesis. The following article describes these methods and how they can be used.

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