Title
RECEIVING OF HAIRY ROOTS FROM FLAX BY TRANSFORMATION OF AGROBACTERIUM RHIZOGENES
Autor
Karolina Cieśla, Sandra Grzegorczyk, Waldemar Rymowicz, Magdalena Wróbel-Kwiatkowska
Keywords
Agrobacterium rhizogenes, flax, gene rolB, hairy roots, transformation
Abstract
The aim of this study was to investigate the efficiency of flax transformation performed via Agrobacterium rhizogenes. The work presents the preliminary data obtained after transformation of flax (Linum usitatissimum L.) of oil (Opal) and fibrous (Nike) cultivars, which was performed with A. rhizogenes strain ATCC 15834. Applied method for transformation was injection of bacteria suspension to flax explants. The results indicate that the used strain of bacteria was virulent to both tested flax cultivars. The highest efficiency of transformation was noticed for Nike cultivar, in which the transformation frequency reached 82%.
Pages
85-92
Cite
Cieśla, K., Grzegorczyk, S., Rymowicz, W., Wróbel-Kwiatkowska, M. (2017). RECEIVING OF HAIRY ROOTS FROM FLAX BY TRANSFORMATION OF AGROBACTERIUM RHIZOGENES. Acta Sci. Pol. Biotechnol., 16(1-4), 85-92.
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