The study evaluated possibilities of using sorghum biomass as a substrate for production of methane in agricultural biogas plants. Five different sorghum varieties, cultivated at two different localisations: Straszków and Śmiłów were analysed. The analysis of biochemical methane potential (BMP) indicated that in both localisations the best results were achieved in the case of Sucrosorgo variety of sorghum. Yield of methane from hectare was 8586 m³ ha^-1 year^-1 in location Straszków, and 7859 m³ ha^-1 year^-1 in location Śmiłów. The lowest yield from hectare and the lowest efficiency of methane from hectare in both locations were registered for the Akklimat variety (2320 m³ ha^-1 year^-1) and Nutri Honey (2762 m³ ha^-1 year^-1). The comparative analysis of the ERoEI index proved that the Sucrosorgo variety is definitely the most effective (8.71 at the Straszków location and 7.97 at the Śmiłów location) from the others in terms of energy efficiency. The energy efficiency of this variety is nearly four times higher than the least effective (Akklimat – 2.34).

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