The physicochemical parameters of the liquid phase have influence significantly on the rate of oxygen absorption in liquid. Feasibility of wave-type agitated single-use bioreactors for bioprocesses involving in vitro cultures makes the study of the influence of liquid properties on values of the volumetric mass transfer coefficient (k a) interesting and actual research issue. ReadyToProcess WAVE^TM25 single-use bioreactor system (WAVE 25; GE Healthcare Bio-Sciences AB, Sweden), equipped with 2 dm³ polymer-based culture bag, has been used for studying the oxygen absorption rate in the wave-induced agitation system. The influence of viscosity of liquid on the rate of oxygen absorption under wave-type agitation has been discussed. The increase of liquid phase viscosity negatively influenced on the rate of oxygen absorption, and lead to decreasing in values of the k_La coefficient in studied system. Fetal bovine serum strongly limited significantly the rate of oxygen absorption into the liquid phase, what resulted in a significant decrease in the k_La coefficient.

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