Erythritol is a zero caloric polyol applied in food industry as a replacement of sucrose. The biosynthesis of erythritol is possible with the use of Yarrowia lipolytica yeast, glycerol and high osmotic pressure. The aim of this study was to design the erythritol separation process from the yeast culture broth and develop the method of utilization of the liquid waste derived from purification process. As a result of two-stage crystallization process the crystals of erythritol with 90.6% of purity were obtained. The liquid waste from crystallization was recycled to biosynthesis process, in which yeast produced 170 g dm^-3 of erythritol, with productivity and yield of 0.9 g dm^-3 h^-1 and 0.57 g g^-1, respectively. Proposed solutions allowed to design the waste free and fully integrated process of erythritol crystals production.

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