The paper presents research on the possibility of using textile wastewater (TW) as a culture medium for microalgae and cyanobacteria. Thermophilic strains of the microorganisms Synechococcus sp. PCC 6715 as well as mesophilic Chlorella sp. 155, Chlorella sp. 179 and isolates from the sewage treatment plants PTx1 and PTx2 were used in the research. The highest production of biomass in 100% textile wastewater demonstrated PTx1. The effects of supplementation TW with NaHCO₃ as an inorganic source of carbon and NaNO₃ as an additional source of nitrogen in reference to BG11 were also studied. The processes were carried out in shake flasks with a working volume of 250 cm³ under both sterile and non-sterile conditions. The highest biomass production was achieved for 100% TW. The Biochemical Methanogenic Potential (BMP) of the biomass was in the range of 372–398 cm³_CH4×g_SMO^-1. The proposed process allows obtaining energy-rich microalgae biomass.

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