Acta Scientiarum Polonorum

Scientific paper founded in 2001 year by Polish agricultural universities

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Biotechnologia
(Biotechnologia) 17 (4) 2018
Title
APPLICATION OF FOAM FRACTIONATION IN FIKOBILIPROTEIN PURIFICATION
Autor
Anna Klepacz-Smółka, Kinga Sołtysiak, Damian Pietrzyk, Stanisław Ledakowicz
Keywords
foam fractionation, phycobiloprotein, cyanobacteria, phycocyanine, Synechococcus PCC6715
Abstract
The paper analyzes the possibility of using foam fractionation to concentrate and purify phycobiliprotein from Synechococcus PCC6715 biomass. The efficiency assessment was made by calculating the efficiency of the process in terms of recovering of phycocyanine and its content in the individual stages of the process. The crude extract was obtained by biomass extractions with PBS buffer using alternate defrosting and thawing cycles. The extract did not require the addition of the surfactants because it showed the ability to form stable foam. The tested method, however, is not characterized by high selectivity, because together with phycobiliproteins other proteins and compounds (e.g. carbohydrates) migrated to the foam fraction.
Pages
49-54
Cite
Klepacz-Smółka, A., Sołtysiak, K., Pietrzyk, D., Ledakowicz, S. (2018). APPLICATION OF FOAM FRACTIONATION IN FIKOBILIPROTEIN PURIFICATION. Acta Sci. Pol. Biotechnol., 17(4), 49-54.
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