The aim of the work was to examine the growth of Y. lipolytica A-101 yeast in batch culture using media containing frying fats. Four different vegetable fats were used as a carbon source: sunflower oil, palm oil, rapeseed oil and modified oil (PM). Frying fats varied by the degree of degradation: fresh fats, used fats after 8h and 40h of snacks frying at 180 ^oC. The yeast of Y. lipolytica A-101 grew on all substrates. The best growing was observed in the medium containing rapeseed oil, giving biomass higher than 28.0 g_d.w · dm^-3 and high biomass productivity from 1.6 to 1.9 g×dm^-3 · h^-1. The biomass yields in processes were quite high and ranged from 0.83 to 0.94 g · g^-1. Those parameters were lower in other cultures and decreased with the degree of degradation of frying fats. Total protein content of yeast and dry matter were similar in all cultures, ranging from 26% for biomass obtained in medium with palm oil to 31% for biomass obtained in medium with rapeseed oil. The content of protein in biomass didn’t depend on the degree of degradation of fats within frying.

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