The aim of the research was to evaluate the stability and kinetics of the process of continuous citric acid (CA) biosynthesis by acetate mutants of Y. lipolytica on fructose syrup in a membrane reactor. Higher yield (YCA= 0,75 gg^-1) and citric acid production rates (Q_CA= 1 gdm^-3h^-1; q_CA= 0,035gg^-1h^-1) were observed in continuous process with the rough A-101-1.31 strain. The process involving its smooth segregant A-101-1.31-K1 showed better stability and enabled to obtain significant quantities of CA. Physiological state assessment of yeasts used in the study showed their good viability and high genetic and phenotypic stability during continuous CA production in membrane reactor with cells recycle.

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