The current study was designed to evaluate the counts of selected caecal microbial populations after addition of humic and fatty dietary supplement Humobentofet (HBF). Ten California White rabbits aged 6 months each were slaughtered. Their caecal contents diluted with buffer were the control samples of the study, and to the second part of the samples was added HBF(1g per sample). The samples were incubated in anaerobic conditions in the shaking water. The total bacterial counts, the counts of cellulolytic bacteria, Escherichia coli, Clostridium perfringens, moulds and yeasts in the caecal content were established at the beginning, 6^th and 24^th hours of fermentation. The decrease of the total bacterial counts, moulds and yeasts was observed in the samples with HBF (6,739 log x g^-1 and 4,637 log x g^-1 caecal content, respectively) in comparison with control (8,187 log x g^-1 and 5,050 log x g^-1) at 6^th hours (P≤0,05). Only small, non insignificant statistically decrease of the number of the cellulolytic bacteria, which are the predominant bacteria in caecal fibre digestion, and E. coli, was observed in comparison with control. The presence of Cl. perfringens was not observed during the experiment.

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