The aim of the studies was the electrophoretic characterization of gliadin and glutenin proteins and evaluation of protein and wet gluten contents in kernels of 4 Triticum durum Desf. lines under conditions of different nitrogen supply. On the basis of electrophoretic separation of gliadin fraction (A-PAGE) it was found that all lines contained γ-45 and γ-42 subunits. Clearly more intensive dying of sub-units on electrophoregrams of gliadins from objects with two times higher nitrogen supply, was observed. Evaluating the percentage of genotype share on the basis of gliadin protein polymorphism, lack of genetic uniformity of studied lines was found. Qualitative assessment of varieties – on the basis of glutenin subunit identification (SDS-PAGE) –revealed that studied lines contained Glu-A1 locus at null combination. Among the studied lines, LGR 899/62a line was characterized with the highest protein and gluten contents. In the case of all the studied lines, higher protein and gluten contents after application of 180 kg N × ha^-1 was observed.

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