The research material was made up of ‘Wanad’ spring triticale grains obtained from the experiments carried out from 1999 to 2001 at the Zawady Experimental Station of the Podlasie University in Siedlce. The aim of the present research was to define the effect of the sowing density (500 and 750 grains per square meter) and herbicides (Arelon 75 WP and Puma Super 069) on the amino acids composition and biological value of the protein (CS and EAAI). The content of exogenous and endogenous amino acids in the triticale grains and the biological value of protein (CS and EAAI) were higher for the sowing density of 750 rather than 500 grains per square meter. The herbicides applied did not show a significant effect on the content of amino acids in spring triticale kernels. The increased precipitation in 2000 enhanced the accumulation of amino acids in triticale grains.

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