The investigation was performed on 1085 Black-and-White cows with a large contribution of the genes of HF breed. The aim of this study was to determine the genetic differentiation degree on the basis of leptin gene polymorphism (LEP/HphI and LEP/Sau3AI). The genotypes analysis was performed using PCR-RFLP method. Frequencies of LEP/HphI genotypes and alleles were as follow: CC – 0.588, CT – 0.356, TT – 0.056, C – 0.766, T – 0.234. Frequencies of LEP/Sau3AI genotypes and alleles were: AA – 0.630, AB – 0.190, AC – 0.140, BB – 0.015, BC – 0.015, and C – 0.010, A – 0.796, B – 0.117, and C – 0.087.

95-106