Abstract. The aim of our study was to find out the prevalence of methicillin resistant Staphylococcus aureus (MRSA) in the live-stock farms in the Czech Republic. 444 samples, obtained from 12 live-stock farms were examined. Samples consisted of animal swabs, milk, feed, feces, swabs from environment and staff. Nine MRSA isolates were found, 8 isolates were obtained from goat’s farm (farm A): 5 isolates from goat’s milk and 3 from staff (nasal swabs). One isolate was obtained from cow’s milk on farm B. All obtained MRSA isolates were genetically characterized. The results from this study revealed that the animals can be an important source of methicillin resistant staphylococci and represent potential hazard for further spread to farmers, staff, their families, other animals and foodstuffs of animal origin.

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